Review



macrophage colony  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 98
      Buy from Supplier

    Structured Review

    MedChemExpress macrophage colony
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Macrophage Colony, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-csf%2C+mouse/M-CSF%2C+Mouse/pmc13471083-120-17-23
    Average 98 stars, based on 35 article reviews
    macrophage colony - by Bioz Stars, 2026-10
    98/100 stars

    Images

    1) Product Images from "APOE -associated lipid-handling macrophages in hepatocellular carcinoma: ligand–receptor communication and host Apoe -linked myeloid remodeling"

    Article Title: APOE -associated lipid-handling macrophages in hepatocellular carcinoma: ligand–receptor communication and host Apoe -linked myeloid remodeling

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2026.1902143

    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.
    Figure Legend Snippet: Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.

    Techniques Used: Isolation, Staining, Flow Cytometry, Fluorescence, Two Tailed Test, Derivative Assay

    Related Articles

    Recombinant:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet

    Incubation:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet

    Cell Culture:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet

    Lysis:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet

    Centrifugation:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet

    Isolation:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet

    Co-culture Assay:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet

    In Vitro:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet

    Polyacrylamide Gel Electrophoresis:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet

    Protein Extraction:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet

    Extraction:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet

    Enzyme-linked Immunosorbent Assay:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet

    Mouse Assay:

    Article Title: Mitochondria-derived peptide hydrogel augments mitochondrial transplantation for promoting cardiac repair via macrophage metabolic reprogramming
    Article Snippet: 56 °C for 30 min; Cat. No. 164210, Procell), 1% penicillin-streptomycin solution (Cat. No. P1400, Solarbio), and 20 ng mL −1 recombinant macrophage colony-stimulating factor (M-CSF, Cat. No. HY-P7085, MCE). Cells were seeded in culture dishes and incubated at 37 °C in a humidified atmosphere containing 5% CO 2 . On day 3, half of the culture medium was replaced with an equal volume of fresh complet



    Similar Products

    macrophage colony  (MedChemExpress)
    98
    MedChemExpress macrophage colony
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Macrophage Colony, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-csf%2C+mouse/M-CSF%2C+Mouse/pmc13471083-120-17-23
    Average 98 stars, based on 1 article reviews
    macrophage colony - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    mouse macrophage colony  (R&D Systems)
    96
    R&D Systems mouse macrophage colony
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Mouse Macrophage Colony, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-csf%2C+mouse/Recombinant+Mouse+M-CSF+Protein/pm42778106-205-0-17
    Average 96 stars, based on 1 article reviews
    mouse macrophage colony - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    factor m csf  (ABclonal Biotechnology)
    95
    ABclonal Biotechnology factor m csf
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Factor M Csf, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-csf%2C+mouse/Recombinant+Mouse+M-CSF%2FCSF-1+Protein/10__31083_slash_fbl53391-65-30-33
    Average 95 stars, based on 1 article reviews
    factor m csf - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    mouse macrophage colony  (ABclonal Biotechnology)
    95
    ABclonal Biotechnology mouse macrophage colony
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Mouse Macrophage Colony, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-csf%2C+mouse/Recombinant+Mouse+M-CSF%2FCSF-1+Protein/pmc13486727-153-31-36
    Average 95 stars, based on 1 article reviews
    mouse macrophage colony - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    recombinant macrophage colony  (MedChemExpress)
    98
    MedChemExpress recombinant macrophage colony
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Recombinant Macrophage Colony, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-csf%2C+mouse/M-CSF%2C+Mouse/pm42570377-143-19-25
    Average 98 stars, based on 1 article reviews
    recombinant macrophage colony - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    murine recombinant m csf  (MedChemExpress)
    98
    MedChemExpress murine recombinant m csf
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Murine Recombinant M Csf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-csf%2C+mouse/M-CSF%2C+Mouse/pmc13400354-321-18-21
    Average 98 stars, based on 1 article reviews
    murine recombinant m csf - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    mouse m csf  (MedChemExpress)
    98
    MedChemExpress mouse m csf
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Mouse M Csf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-csf%2C+mouse/M-CSF%2C+Mouse/pmc13050104-39-30-32
    Average 98 stars, based on 1 article reviews
    mouse m csf - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    mouse mce cat  (MedChemExpress)
    94
    MedChemExpress mouse mce cat
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Mouse Mce Cat, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-csf%2C+mouse/M-CSF%2C+Mouse/pm42507550-262-202-203
    Average 94 stars, based on 1 article reviews
    mouse mce cat - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    m csf  (MedChemExpress)
    98
    MedChemExpress m csf
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    M Csf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-csf%2C+mouse/M-CSF%2C+Mouse/pmc13400143-34-0-2
    Average 98 stars, based on 1 article reviews
    m csf - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    m csf  (ABclonal Biotechnology)
    95
    ABclonal Biotechnology m csf
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    M Csf, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-csf%2C+mouse/Recombinant+Mouse+M-CSF%2FCSF-1+Protein/pmc13374752-94-0-2
    Average 95 stars, based on 1 article reviews
    m csf - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.

    Journal: Frontiers in Immunology

    Article Title: APOE -associated lipid-handling macrophages in hepatocellular carcinoma: ligand–receptor communication and host Apoe -linked myeloid remodeling

    doi: 10.3389/fimmu.2026.1902143

    Figure Lengend Snippet: Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.

    Article Snippet: Bone marrow cells from WT and Apoe −/− mice were differentiated into bone marrow-derived macrophages (BMDMs) with macrophage colony-stimulating factor (M-CSF; 25 ng/mL; MedChemExpress, HY-P7085) for 7 days.

    Techniques: Isolation, Staining, Flow Cytometry, Fluorescence, Two Tailed Test, Derivative Assay